Study on thawing methods of straw frozen semen in cashmere goats

The semen of six 2-year-old liaoning cashmere bucks were collected and mixed to gain some 0.25 mL straw frozen semen. Three thawing methods (37 ℃ 12 s, 39 ℃ 8 s, 70 ℃ 4 s) were respectively applied to thaw the frozen semen as mentioned above. Sperm vitality, sperm survival time, acrosome intact rate, deformity rate, enzymes in seminal plasma of post-thawed semen and the heating curve were analyzed to study the effects of the thawing methods on sperm quality of straw frozen semen in cashmere goats. The results show that, sperm vitality of the frozen semen after thawing for 4 s under 70 ℃ was significantly higher than that of 12 s under 37 ℃ (P0.05), and it was higher than that of 39 ℃ 8 s which was not significantly (P0.05); There were no significant difference between the survival time of the thawed sperm among three thawing methods (P0.05); the intact rate of sperm acrosome thawed with 70 ℃ for 4 s was significantly higher than that of the other two thawing methods (P0.05), while the sperm deformity rate thawed with 70 ℃ for 4 s was significantly lower than the other two thawing methods of (P0.05); the GOT activity thawed with 70 ℃ 4 s was significantly lower than that of the other two thawing methods (P0.05), and the LDH activity and HYD content thawed with 70 ℃ 4 s was significantly lower than that of 37 ℃ 12 s (P0.05); the semen thawed with 70 ℃ 4 s went through the danger zones at the highest velocity. Therefore, the thawing method of at 70 ℃ for 4 s is suitable for thawing straw frozen semen of cashmere goats.