A study on transformation of Hypodermin A gene into Medicago sativa

The high efficiency regeneration and genetic transformation system of alfalfa （Medicago sativa）was established by using the cotyledons as explants from the sterile 7days old seedlings of M.sativa cv. Gannong No.1. The optimal medium for callus induction and regeneration from Gannong No.1 cotyledons was MS+2,4D 2.0 mg/L+6BA 0.5 mg/L and MS+6BA 0.5 mg/L+NAA 0.03 mg/L+GA3 2.0 mg/L respectively. In this study, the efficiency transformation system included agrobacterium concentration OD6000.5, infection time 8-10 min and coculture time 2 d. The transgenic plants were identified by PCR and Southern blot techniques. The results indicated that the Hyperdomin A （HA） gene had been transformed into the genome of Gannong No.1 alfalfa.