Cloning and function analysis of MvP5CS in Medicago varia xinmu1

The MvP5CS of Medicago varia Xinmu1 was cloned by RTPCR and homology cloning. The MvP5CS was 2 148 bp long and the accession number of Genbank was EU371644. A Realtime PCR assay showed that the level of MvP5CS transcriptions was obviously upregulated in the seedlings after longer time salinity treatment, indicating that MvP5CS was related to salt tolerance. The MvP5CS genes of M. varia Xinmu1 was transferred successfully into tobacco via agrobacterium mediation. The germination percentage and root length of T1generation transgenic tobacco were higher than that of nontransgenic tobacco under salt stress, which indicated that the MvP5CS enhanced the salt tolerance of transgenic tobacco, and this provide references for salttolerance molecular mechanism and future stressresistance molecular breeding of M.sativa.