Primary study on preparation procedure of paraffin section with embryo structure of Poa pratensis

A study was conducted to understand embryo structure of Poa pratensis with paraffin section. The effects of Sarranine-Fast green and Ehrlich’s hematoxylin-eosin double stains with different dyeing time were analyses to improve the quality of paraffin section. This study selected better preparation procedure is as following: 95% eosin alcohol was selected instead of 95% alcohol for dehydration; multisegment dimethybrnzene was used instead of ethyl alcohol for transparent; rising temperature along with more wax gradually for wax dipping; slices were flatted and taken out in 40 ℃ warm water bath and other improvements during procedure. Based on these improvements, better quality paraffin sections were achieved with dyeing with Ehrlich’s hematoxylin-eosin double stains for 30 min and returning blue with water for 30 min.