Establishing regeneration systems of Gannong No.4 alfalfa

Hypocotyls of Gannong No.4 alfalfa were used as explants, MS was used as the basic medium, different concentration of 2,4D and 6BA were added into the medium to induce the callus. The rate of inducement ranged from 86.85% to 99.56%, the treatment of MS+2 mg/L 2,4D + 0.5 mg/L 6BA had the highest inducement rate; yellow and green callus had best differentiation on the medium of MS+0.5 mg/L KT+0.1 mg/L NAA, and the color of plants was tenderly green. The regenerated plants were obtained in the root inducing medium (1/2MS+0.5 mg/L NAA).