Analysis on seed storage protein of Lespedeza hedysaroides

Seed storage proteins of three Lespedeza hedysaroides were analyzed by SDSPAGE technique. Results indicated that SDSPAGE of saltsoluble proteins and seed storage proteins were practical and reliable methods for determining relationships among three L.hedysaroides. Saltsoluble protein and seed storage protein in the commontype and deep greentype L.hedysaroides were significantly different from those in the talltype. The protein molecular weight ranged from 10.375 KDa to 155.53 KDa. 6 bands were shared and 13 bands were polymorphism (68.42%) of total 19 saltsoluble protein bands. 19 bands were shared and 9 bands were polymorphism (32.14%) of total 28 seed storage protein bands. Clustering showed that three L.hedysaroides were divided into two groups. The commontype and the deep greentype were classified as the first group and the talltype one was second group. Therefore, SDSPAGE technology is an important method to study intraspecies variation among Lespedeza species.