Effects of external hormones on induction and differentiation of callus of Chinese pennisetum

The base of young shoots of Pennisetum alopecuroides was used as explants in this experiment to determine the effect of external hormones on induction and differentiation of callus of Chinese pennisetum by screening appropriate hormone type and concentration ratio. The results of this study showed that the 2, 4D was significantly effective for callus induction. The effect of hormone NAA and KT on growth was similar and their appropriate concentration was 0.5-1.0 mg L-1. The NAA was effectively useful for callus differentiation under combination 0.5-1.0 mgL-1 NAA and 3.0 mgL-1 6BA, but KT did not work on callus differentiation. This study suggested that the best induction culture medium was MS+2, 4D 3.0 mgL-1+NAA 1.0 mgL-1 +KT 1.0 mgL-1, and the best differentiation culture medium was MS+NAA 0.5 mgL-1 +6BA 3.0 mgL-1, which would provide information for resistance breeding of Pennisetum clones.