RAPD analysis of different major cultivated species of Codonopsis pilosula in Gansu

Genetic differences and relationship of 10 (8 lines and 2 cultivars) Codonopsis pilosula species in Gansu were examined by RAPD in this study. The results showed that a total of 159 DNA bands were amplified by 14 oligonucleotide primers, of which 26 bands were polymorphic. The rate of polymorphism was 45.61%. According to the cluster analysis, the 10 C. pilosula species had genetic similarity coefficients ranged from 0.771 9 to 0.964 9. The genetic relationship of DS6 species was far from others. However, the genetic relationship between DS3 and DS8 was close with a similarity coefficient of 0.964 9.