Seed dormancy characteristics and germination condition of Tulipa gesnersiana

In view of problems such as Tulipa gesneriana seed could not germinate in room temperature, and it had slowly germination process and low germination rate, seeds was liable to moldy and so on in cold storage, so some key control tests such as piercing the seeds and improving the bud beds were utilized to study the germination condition in room temperature on the basis of reported experiment.The results showed that the wild T.gesneriana seed had deep dormancy phenomenon, and the dormancy could be absolved by low temperature, GA3 treatment, puncturing seeds, improving the bud beds et al, but light and room temperature storage had not significant effects to the breaking of seed dormancy. During the germination procession, there was a longer germinating imbibition period for T.gesneriana seed, and it showed a distinct sequence of germination behavior. At room temperature, the current year T.gesneriana seed which by frozen for 15 d after storage 6 months at 4 ℃, it was punctured and put on the medium of 0.5 mg/L GA3 concentrations in continued light, and the germination rate was 25%, 50% and 95% respectively, after 10 d, 30 d, 45 d and 50 d, while under the same conditions, the germination rate of unpunctured seeds was very low. The germination rate was no significant difference at the frozen temperature between 4 ℃ and -10 ℃. Puncturing seeds could improve the germination rate significantly, in an extent, colloidal endosperm restrained the growth of embryo of T.gesneriana. Budbed was an important role in the germination of seeds, it was not only guaranteed the supply of nutrients during the germination of the seeds, but also kept the continuing effect of GA3 on seeds to use the culture medium as budbed, however, paperbed was often contaminated and kept from the seed germination.