Isozyme band analysis of Chinese Iris germplasms

POD isozyme banding patterns of were identified for 23 wild Chinese Iris germplasms by means of vertical polyacrylamide gel electrophoresis. This study showed that the relative mobility (Rf) located at 0.041 to 0.515 loci, and the band numbers ranged from 5 to 9, in which BJCYML004 and BJCYML020 band numbers were the most (9), BJCYML001、BJCYML018、BJCYML021、BJCYML029 band numbers were the least (5). The genetic similarity of POD ranged from 0.516 to 1.000, and the genetic similarity between BJCYML004 and BJCYML020 was the lowest (0.516) and those between BJCYML028 and BJCYML031 was the highest (1.000). The genetic similarity of other germplasms was from 0.548 to 0.968, indicating that there were close genetic similarity of all germplasms. Cluster analysis showed that the 23 wild Chinese Iris were classified into 4 firstclass groups when the genetic similarity was 0.71, suggesting that the Iris lactea var. chinensis communities were related to their geographical distribution and ecological habitats.