Effects of Nacl on the tissue culture of creeping bentgrass and antioxidant enzyme activities

Effects of different NaCl concentration(0,17,51,85,136,204 and 272 mmol·L-1)on creeping bentgrass (Agrostis soionifera) seed germination rate, callus formation, growth, plantlet regeneration and POD and SOD activities of regenerated seedlings were studied using creeping bentgrass seeds. The results showed that creeping bentgrass germination rate, callus induction rate and differentiation rate and seedling regeneration rate decreased with NaCl concentration increasing gradually. When NaCl concentration was above 272 mmol·L-1,seed germination was inhibited completely. When NaCl concentration was 204 mmol·L-1, calluses were not able to grow at all. Under salt stress, SOD activity of creeping bentgrass regenerated seedlings rose to the maximum value of 83 U·g-1 and then declined. Changes of POD activity were similar to SOD activity.