Detection methods of pollen viability of wild Dichondra repens

The pollen viability of wild Dichondra repens (SD200303 and GD200503) was detected by methods of I2KI, 4, 4diaminobiphenyl1Naphthol, TTC and pollen germination in vitro, respectively. The comparison of the four methods indicated that the methods of I2KI and TTC were not suitable to evaluate wild Dichondra repens pollen activity. 4,4diaminobiphenyl1Naphthol method could detect the pollen viability, but the measured value was higher than that of pollen viability. The method of germination in vitro was the fittest and convenient, in which pollen tube grew well and pollen viability was stable. The optimum culture medium was 10% Sucrose＋0.005% HBO3＋0.015% Ca(NO3)24H2O ＋0.001% MgSO47H2O＋0.005% KNO3 ＋0.5%Agar.