Comparative study on extraction methods of total RNA from creeepingrooted alfalfa

Total RNA from root, stem and leaf of Creeepingrooted alfalfa were extracted with four methods, including a modified CTAB method, TRNzol method and other two methods, and its quality and quantity were compared. The results indicated that the modified CTAB method was better than the original method since the value of OD260 nm/OD230 nm and OD260 nm/OD280 nm were 2.0 and 1.8, the purities were great higher, the bands of 28S and 18S rRNA on Gel eletro pH oresis were very clear and did not degradated. Comparably, TRNzol method was not very good as the first method because RNA was easy degraded and dispersed although the value is higher, and other two methods were not fit the requirement of RNA extraction.