The protocol for processing of the chromosomal images of Miscanthus for karyotype analysis using Adobe Photoshop software

tudy of plants of Miscanthus is one of the topics of keen interest worldwide because of their growth habits, huge potential utilization. So it is regarded as a alternative bioenergy plant to replace of grain crops. Karyotype analysis is important to the relevant research about Miscanthus, and chromosomal image processing is an important link in the process. This report was to optimize the key steps for processing of chromosomal images for karyotype analysis in some taxa of Miscanthus based on Adobe Photoshop software, including light exposure compensation, background preliminary elimination, removal of chromosomal images, color contrast adjustment, rearrangement of chromosomal image, separation of the crossed chromosomal region, and homologous chromosomes pairing. The images were pretty based on our methods. This report also provides a reference for chromosomal image processing in other plant species.