Study on induction of callus from mature embryo andplant regeneration of Zoysia japonica

The embryo of Zoysia japonica was used as explant to induce the embryogenic callus and then regenerate the plant. The result showed that the pretreatment method remarkably affect embryogenic callus induction and the ratio of embryogenic callus induction was 82.67% by removing hull, 30% NaOH soaking for 1 min, then rinsing 30 min with tap water. The basal MS media was supplemented with 3 mg/L of 2,4D and 0.1 mg/L of 6BA. The quality and quantity of embryogenic calli were obviously improved and increased respectively by 1 to 2 rounds reculture on the basal MS media supplemented with 2 mg/L of 2,4D and 6BA (0.2 and 0.5 mg/L). Embryogenic Calli were inoculated on the basal MS media supplemented with 1 mg/L of KT and 0.1 mg/L of NAA and the ratio reached 86%. The rooted plantlet clusters could be easily transplanted and the survival rate was 100%.