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Crystalloid loline dihydrogen chloride alkaloids from meadow fescue were purified by a series of extractions and reextractions. 8 kinds of different structural alkaloids with different bioactivities had different insecticidal properties. Concentration of loline in gramineous plants varied in different growth periods and affected the insecticidal activity. Therefore, the content of loline in leave and stem of meadow fescue from New Zealand, which was infected by endophytes with 12 different methods, was measured for selecting endophyte and grass combinations with high yield, longevity and insecticidal properties.