Tissue culture and rapid propagation of Orostachys malacophyllus leaves with axillary buds

The leaves with axillary buds of Orostachys malacophyllus were selected to investigate the effect of different plant growth regulators on axillary buds initiation by using MS medium with different concentrations of 6benzylamino purine, 1naphthaleneacetic acid, and 2,4dichlorophenoxyacetic acid for developping an efficient procedure of plant regeneration of O. malacophyllus. The results of this study showed that the optimum initiating culture medium for leaves axillary buds of O. malacophyllus was MS medium appended with 3 mgL-1 6benzylamino purine and 0.1 mgL-1 1naphthaleneacetic acid. The optimum proliferative medium for axillary buds of O. malacophyllus was MS medium added with 1.5 mgL-1 6benzylamino purine and 0.02 mgL-1 2,4dichlorophenoxyacetic acid, and multiplication times was 5.35. In root induction culture, shoot clusters in the1/2 MS medium supplemented with 0.3 mgL-1 1naphthaleneacetic acid were well rooted and grew vigorously with the 96.67% rate of root formation, and the best survival rate was 76.67%. This study suggested that the efficient plant regeneration in O. malacophyllus from leaves with axillary buds was possible.