Application status of chemical hybridizing agent in plant breeding

The research results on chemical hybridizing agent were summarized, which included the mechanism and existing problems of chemical hybridizing agent and its application possibility in forage hybridization breeding. The developing of chemical hybridizing agent experienced three stages such as research on growth regulator induced male sterility, exploitation new chemical hybridizing agent compounds and application of chemical hybridizing agent in plant breeding. The mechanism of chemical hybridizing agent was not very clear at present, the information showed that EXP of chemical hybridizing agent could restrain the synthesis of branchedchain amino acids valine, leucine and isoleucine in vivo of rape, finally lead to male sterility. SC2053, BAU2, GENESIS could induce male sterility by declining pollen viability on wheat, decreasing anther proline content and enzymic activity and so on. Chemical hybridizing agent had few applications on the forage breeding, they mainly focused on the discussion of the emasculation effective, however few study were about getting the forage hybrid seeds by this method.