Studies on total RNA extraction methods from Chorispora bungeana callus

This research was aimed to find the optimum methods and conditions to extract total RNA from Chorispora bungeana. Three RNA extraction methods (Improved Trizol, Kit and CTAB) were compared by yield, purity, integrity and PCR amplification effect after reverse transcription. The improved Trizol method was proved to be the best to extract total RNA from C.bungeana. RNA can be preserved for 8 h at room temperature and more than 30 days at -80 ℃. A large amounts of RNA would be degraded if RNase in tips, tubes was incompletely removed, and the incomplete RNase inactivation in electrophoresis tank and solution would cause RNA degradation during electrophoresis.