Stamen dynamics and pollen fertility of Allium obliquum in Xinjiang Province, China

Allium obliquum only distributes in the Xinjiang Province, China. The marked plants were used to observe stamen morphological characteristics to analyze the floral dynamics of stamen and test pollen fertility of A.obliquum by 6 methods. This study showed that five morphological phases were found during flowering period. The inner filaments earlier expanded than the outer ones, and the inner anthers firstly expanded from perianth. It was about 4-5 d for all 6 anthers from open to fall. The individual anther released pollen about 1.5- 2.0 h from the beginning dispersion to the end. Pollen viability kept about 0-10 h above 50% after anther dehiscence. The triphenyl tetrazolium chloride, diphenyl tetrazolium bromide, benzidine, and the in vitro germination test were used to test pollen fertility and the acetocarmine and iodine potassium iodide test was not suitable for A.obliquum pollen testing. A.obliquum was dichogamy and protandry with fertility, short longevity pollen, which would provide basic theory and method for pollen development, pollination biology and artificial sexual reproduction by and six methods were used to test pollen fertility.