A study on genetic diversity of Buchloe dactyloides with different geographical origin

30 Buchloe dactyloides collected from different geographical origins were put into this research. The basic turf characters, including leafblade length, leafblade width, height, diameter, number of tillers, number of stolon, length of stolon inter node, length of stolon, leaf color, extent of leaf turning yellow were measured and the genetic diversity was analyzed using the ISSR technique. The following results were obtained: 1) The difference of leaf color among these germplasm was significant (P0.05）, and the difference of leafblade length, leafblade width, height, diameter, length of stolon, number of stolon was very significant (P0.01). 2) Seven primers produced 108 polymorphic bands, with an average of 15.6 bands per primer. The percentage of polymorphic bands in average was 82.6%. 3) The Neis genetic similarity coefficient (GS) of the tested accessions ranged from 0.546 to 0.88, which suggested that there was rich genetic diversity among the germplasm of B. dactyloides. 4）These accessions were clustered into five groups. Moreover, there was the positive correlation between the geographical distance and the genetic distance.