Cloning and analysis of two promoters of stressrelated genes in Medicago varia Xinmu1

The expression level of MvNHX1 and MvDREB1 of Medicago varia Xinmu1 after salinity treatment was analyzed by using Realtime PCR. The specific primers were designed according to the sequences of MvNHX1 and MvDREB1 obtained in a previous study. The promoters of two genes were cloned by PCR using the total DNA of M.varia Xinmu1 as template. The types and structures of two promoters were analysed by bioinformatics incorporated with the Realtime PCR. The results showed that both of two sequences included common cisacting element and core promoter element, such as CAATBox, TATA Box, light responsive element, low temperature responsive element and so on. However, the types and quantities of several responsive elements were different. The cloning, analysis and comparation of two promoters lay foundations for the further research of the transcriptional regulation mechanism of two genes.