Effects of explants and nitrogen concentration on callus induction of Glycyrrhiza uralensis

An experiment was conducted to determine the effects of different explants and nitrogen concentration on callus induction of Glycyrrhiza uralensis. The tissue culture method was used to compare the effects of root, stem, cotyledon and hypocotyls on callus induction and growth. The results of this study showed that the callus of hypocotyls was induced by higher induction rate, quicker induction time and lower browsing rate, indicating that the hypocotyls was optimal explants to induce the callus. The different concentration of nitrogen had the most significant effects on callus growth and induction of hypocotyls in certain hormone concentration, in which the lower and higher nitrogen concentration had negative effects on the growth of callus, and the optimal nitrogen concentration was 60 mmolL-1, which had the best effects on callus growth and induction. The different explants and nitrogen concentration were the important factors affecting the growth and induction of callus.