Analysis on Cbanding and silver staining karyotypes of Locusta migratoria manilensis

The improved droppingmethod was used to make chromosome slides of male Locusta migratoria manilensis for studying the karyotypes of Cbanding and sliver staining of L. migratoria manilensis. The result of Cbanding showed that all chromomsomes of L. migratoria manilensis were telocentric chromosomes.Telomeric band was detected on chromosme 2. Karyotype of male L. migratoria manilensis was 2N=22+XO(♂). The genomestyle was 4L+4M+4S+XO. AgNOR was only observed on one of chromsome 2 and located on the secondary constriction near the telomere. Chromsome 2 had abundant bands and could be used as the genetic marker of L. migratoria manilensis.