Cloning and expression analysis of transcription factor from Agropyron cristatum

Primers were designed based on the conserved region of cbf, derb, myb in Gramineae plants. RTPCR was used to clone transcription fragments from Agropyron cristatum leaf and 3 fragments of 548 bp, 862 bp, 319 bp were firstly obtained and named as ACcbf，ACdreb，ACmyb, respectively. Semiquantitative RTPCR was conducted to test the expression mode of the three genes under cold and drought stress. The result of this study showed ACcbf was sensitive to cold stress, and more or less, responsed to drought, and ACdreb was sensitive to both drought and cold, and ACmyb was sensitive to drought, but insensitive to cold.