Cloning and sequence analysis of outwardrectifying potassium channelSKOR gene fragment from halophyte Puccinellia tenuiflora

To investigate molecular mechanism of K+/Na+ slective transport in Pucciinellia tenuiflora，degenerate primers were designed based on the conserved sequences of the outwardrectifying potassium channel from other plants． Total RNA was extracted from the roots of P.tenuiflora. SKOR gene fragment was obtained by reverse transcription polymerase chain reaction (RTPCR)，which could provide basis for cloning fulllength SKOR gene，expression regulation and RNAi． The sequencing result showed that the SKOR gene fragment from P.tenuiflora contained about 555 bp，encoding 185 amino acids． Homology comparison with SKOR gene sequences of other higher plants showed that it shared over 66% nucleotide sequence homology and 55% amino acid sequence homology．