Effect of low temperature and plant growth regulators on callus induction and regeneration of Zoysia japonica cv. Qingdao

The stem apex, stem node and root cusp were used as the explants to study the effect of plant growth regulators and low temperature pretreatments on callusing and regeneration of Zoysia japonica cv. Qingdao. The results showed that 3 times (30 days) of low temperature pretreatment (10 ℃) was the best and the highest callus induction rate could be obtained from the 3 types of explants, in which, the stem nodes was the best and its callus induction rate reached 94%, 38% higher than CK and the regeneration rate was 74.47%. As for, , all of the 3 types of explants got the highest rates of callusing and regeneration on the culture medium of MS+2,4D(2 mg/L)＋6BA(0.1 mg/L), and the stem node was also the best, its callusing rate was 94%.