A study on transformation of fused gene HBcAg of FMDV epitopes into Medicago sativa

Abstract: The fused gene O21O14A21HBcAg that contained both type O and A of FMDV epitopes were introduced into alfalfa （Medicago sativa cv. Gannong No.1 ）leaf explants via Agrobacterium tumefaciensmediated transformation. After infection and coculture resistant plants were obtained from the resistant callus and the genetic transformation and regeneration system of alfalfa were established. The GUS reporter gene was transitorily expressed in transformed callus. 52 regenerated individual plants were acquired through Hygromycin screening test. After PCR tests for transgenic plants, four out of seven randomly selected plants were positive. The results of PCR indicated that the O21O14A21HBcAg gene was integrated into the genomes of transformed alfalfa plants.