Preliminary study on callus induction and regeneration of 54Q53 lucerne

In order to establish the high efficiency alfalfa （Medicago sativa） regeneration system for callus induction of the 54Q53 lucerne, two different cytokinins, three explants, and four basic solidmedia were conducted and concentration combination of the auxin and cytokinin was optimized. The results of this study showed that the best protocol was the hypocotyls as the explants and MS medium with plant hormones, 2.0 mg/L 2,4D+0.5 mg/L 6BA, as the induction medium. Furthermore, preliminary alfalfa callus regeneration was studied by using the MS or B5 basic medium supplemented with different concentrations and combinations of KT, NAA. More and stronger adventitious roots were obtained on the MS medium containing 0.5 mg/L KT and the root development would be repressed by adding the NAA.