Effects of simulated warming and fertilization on activities of soil enzymes in alpine meadow

To investigate effects of warming and fertilization on soil enzyme activities in alpine meadows, a simulation study was conducted in situ with OTCs (opentop chambers) method. Results revealed that with warming, activities of soil cellulase and phosphatase increased by 12.4%, 29.1% respectively; urease activity decreased by 18.0%, which suggested that warming could accelerate the C and P cycles in the ecosystem. Under unchanged temperatures, fertilization reduced the activities of soil cellulase, polyphenol oxidase and peroxidase but NPK fertilizer applied raised the activities of soil urease and phosphatase. Reversely, there was no significant effect of fertilization on the activities of soil cellulase, polyphenol oxidase and peroxidase with warming; soluble carbon （Glu） fertilizer applied increased soil urease activity but NPK declined phosphatase activity. In addition, the interaction between warming and fertilization had a significant effect on many kinds of soil enzyme activities. In conclusion, compared with unchanged temperatures, simulated warming did not induce significant differences in the activities of soil cellulase, polyphenol oxidase, peroxidase and urease with fertilization. Amidst the background of climatic warming in the future the response of many kinds of soil enzyme activities to different fertilizers would become inertia in the alpine ecosystem.