Standardization of germination and accelerated aging testing of the Indigofera pseudotinctoria seeds

In order to standardize the testing method of germination of Indigofera pseudotinctoria seed, an experiment was conducted to determine the optimum condition for seed germination and accelerated aging test. In the germination test, the temperature treatments were adopted including 15-25 ℃, 15-30 ℃, 15-35 ℃, 20-30 ℃, 20-35 ℃, 25-35 ℃, 20 ℃, 25 ℃, 30 ℃ and 35 ℃, 2 light treatments(8 h lightness+16 darkness and 24 h darkness). In the accelerated aging trial, ageing temperature were 39 ℃, 41 ℃, 43 ℃, 45 ℃ and ageing duration were 24 h, 36 h, 48 h, 60 h, 72 h, 84 h, 96 h. The results showed that different combination of light and temperature treatments influenced germination rates of I. pseudotinctoria seed. Optimum conditions for seed testing of germination was constant temperature 25 ℃ or alternate temperature 20-30 ℃, 8 h light will be helpful for seedling growth, while total dark condition leads to the etiolated seedlings. There was an interaction effect between temperature and lightness. The first count time is the 7 th day after seed germination, and the last time is the 14 th day for the seedling evaluation. The optimal temperature of accelerated aging treatment was 43 ℃ and the ageing duration was 72 h and there was an interaction effect between accelerated aging temperature and duration.