Effect of different treatments on seed germination of Kobresia bellardii

The study on the seed germination of Kobresia bellardii under different temperature (5, 10, 15, 20 and 25 ℃) and different concentration of three chemical solutions (NaOH, GA and CaCl2) was conducted. The result indicated that the temperature and three chemicals and concentration had significant effect on seed germination rate, germination index and shoot length (P＜0.001). Moreover, the temperature contributed more to the seed germination than the chemicals. The germination temperature range was from 10 to 25 ℃, but the growth of shoot, germination rate and germination index were higher at 20 and 25 ℃ than those at 10 and 15 ℃. The higher chemical concentration promoted the seed germination rate and germination index at lower temperature, however, the lower chemical concentration was favorable to the seeds germination at higher temperature. Under 25 ℃, the best treatment was to soak the seeds in CaCl2 solution with a concentration of 5 g/L, the germination rate was highest and could reach 84%. At 20 ℃, to deal with the seeds in NaOH solution with a concentration of 20 g/L, the highest germination index (5.26) could be reached.