Influence of different hormone combinations on Kentucky bluegrass ‘ABBY’ Callus induction

The present study describes callus induction of the plant ‘ABBY’ Kentucky bluegrass (Poa pratensis) using mature seeds as explants and the influence of different combinations of hormone: 2,4-D ichlorophenoxyacetic acid (2,4-D) and 6-Benzylamin (6-BA) with Murashige Skoog Basal Medium (MS) as the basic medium on callus initiation and subsequent growth. The results showed that the hormone combination: MS+1.0 mg·L-12,4-D+0.2 mg·L-16-BA has highest callus induction rate(54.74%). The hormone combination MS+1.0 mg·L-12,4-D+0.4 mg·L-16-BA has the best quality callus although the callus induction rate is lower(45.81%). The hormone combination: MS+3.0 mg·L-16-BA+0.2 mg·L-1NAA was suggested to be the best differentiation medium with a callus differentiation rate at 100% after 2~3 generations. The differentiation rate was affected by callus condition and incubation time.