Effects of different factors on fatty acid oxidation activity of thyme

Plantlets of thyme were cultured on the 1/2 MS medium that containing surcose with different concentrations, NO3-/NH4+, phenylalanine, glutamic acid and pyruvic acid. The LOX activity of thyme was tested after 30 d. Furthermore, the plantlets were induced for 0～120 h by MeJA, SA and chitosan at different culture for 20 d, 30 d and 40 d, and LOX activity was tested. The results showed that the highest LOX activity was obtained when the plantlets of thyme were cultured on the 1/2 MS medium with 4% sugar and 2∶1 NO3-/NH4+ ratio for 30 d. The LOX activities were increased respectively when adding 0.6 mmolL-1 precursor regulation Lphenylalanine, 0.4 mmolL-1 glutamic acid. The LOX activities of plantlets cultured for 20 d were 655.4％, 503.8％ and 429.1％ more than control by adding 1.0 mmolL-1 MeJA,150 mgL-1 SA, and 200 mgL-1 chitosan. The LOX activities of plantlets cultured for 30 d were increased to 688.1％, 383.8％ and 383.3％ when adding 0.5 mmolL-1 MeJA, 150 mgL-1 SA, and 200 mgL-1 chitosan, respectively. The LOX activities of plantlets cultured for 40 d did not change. Therefore, the LOX activity was affected by adding different inducer at different time.