Study on constructed AtPCS1 plant expression vector and transformation of Medicago sativa

The full length of AtPCS1 gene was amplified from Arabidopsis thaliana (ecotype Columbia), and AtPCS1 plant expression vector pBI121AtPCS1 was constructed. Furthermore, the expression vector was transferred into Agrobacterium EHA105, and AtPCS1 was transferred into alfalfa (Medicago sativa) Gannong No.1 by leaf infection method. Fiftyseven transgenic alfalfa plants have been obtained after a period of 80-100 d after transformation.Six in nine random chosen plants were positive transgenic plants identified by AtPCS1specific PCR. Results from this study showed that AtPCS1 has been transferred into genome of alfalfa.