The study of the method for isolation and purification of ct DNA from turfgrasses

Four turfgrass including Festuca elata, Lotium perenne, Cynodon dactylon and Zoysia sinica were selected as the materials, the chloroplast was isolated by improved differential centrifugation, and the ctDNA was extracted with the CTAB and tested by Agarose gel electrophoresis. The results showed that the purity of ctDNA was high, the bands were clear, the ratio of intact chloroplast was high with the improved CTAB method.Compared with traditional method of ctDNA isolation and purification, CTAB method eliminated the steps of consuming material and time in traditional density gradient centrifugation; it was simple, rapid and high yield. So the improved CTAB method provided a reference for the extraction of Gramineae plants ctDNA, which was applicable for biological experimental teaching and research activities.