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北细辛悬浮培养体系的建立及优化

刘思妤, 杨悦, 王鹰, 王丽娟, 吴秀菊

刘思妤, 杨悦, 王鹰, 王丽娟, 吴秀菊. 北细辛悬浮培养体系的建立及优化[J]. 草业科学, 2017, 11(11): 2254-2260. DOI: 10.11829/j.issn.1001-0629.2017-0029
引用本文: 刘思妤, 杨悦, 王鹰, 王丽娟, 吴秀菊. 北细辛悬浮培养体系的建立及优化[J]. 草业科学, 2017, 11(11): 2254-2260. DOI: 10.11829/j.issn.1001-0629.2017-0029
Si-yu Liu, Yue Yang, Ying Wang, Li-juan Wang, Xiu-ju Wu. Establishment and optimization of a suspension culture system for culturing Asarum heterotropoides[J]. Pratacultural Science, 2017, 11(11): 2254-2260. DOI: 10.11829/j.issn.1001-0629.2017-0029
Citation: Si-yu Liu, Yue Yang, Ying Wang, Li-juan Wang, Xiu-ju Wu. Establishment and optimization of a suspension culture system for culturing Asarum heterotropoides[J]. Pratacultural Science, 2017, 11(11): 2254-2260. DOI: 10.11829/j.issn.1001-0629.2017-0029

北细辛悬浮培养体系的建立及优化

基金项目: 

国家基础科学人才培养基金能力培养与科研训练项目(J1210069)

黑龙江省教育厅科学技术研究项目计划(12531030)

哈尔滨市科技局科技创新人才研究专项(2012RFLXN003)

摘要: 以北细辛(Asarum heterotropoides)幼嫩叶柄诱导的愈伤组织为材料,初步建立悬浮培养体系,同时探讨接种量、光照、培养液量、转速、蔗糖浓度、激素配比等因素对细胞生物量的影响。结果表明,100 mL的1/2MS液体培养基中,2 g愈伤组织是最适接种量,蔗糖浓度为30 g·L-1,摇床转速150 r·min-1,0.6 mg·L-1 6-BA+0.3 mg·L-1 NAA,全天光照培养时细胞生长量最大。北细辛悬浮细胞生长曲线大致为“S”型,悬浮细胞的最佳收获周期为15 d。经悬浮培养后可快速获得细胞团及多种形态的单细胞,可为北细辛悬浮细胞的大规模生产提供依据,并进一步提升北细辛的应用价值。

 

English

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  • 收稿日期:  2017-01-06
  • 发布日期:  2017-11-09

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