多花黑麦草绿汁发酵液中乳酸菌的分离鉴定

李季; 杨春华; 贾露洁; 梁欢; 郭丽娟; 刘羽; 李攀峰

多花黑麦草绿汁发酵液中乳酸菌的分离鉴定

四川农业大学动物科技学院,四川雅安 625000

基金项目:

国家科技支撑计划项目(201lBADl7800)

摘要: 为研究在多花黑麦草(Lolium multiflorum)青贮中起主要作用的乳酸菌种类,从长江2号多花黑麦草调制的绿汁发酵液中分离得到6株乳酸菌(L-1、L-3、L-4、L-5、L-8和L-9)。经传统生理生化鉴定、产酸性试验、耐酸性试验、耐盐性试验及16S rDNA序列分析法最终确定,菌株L-1为植物乳杆菌(Lactobacillus plantarum),菌株L-3为乳酸乳球菌(L.lactis),菌株L-4为乳酸球菌(L.garvieae),菌株L-5为乳酸明串珠菌(Leuconostoc lactis),菌株L-8为魏氏菌(Weissella confusa),菌株L-9为魏氏菌(W.cibaria),且都具有良好的产酸性、耐酸性以及耐盐性,可进一步研发,作为青贮饲料添加剂。

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English

Isolation and identification of lactic acid bacteria from previously fermented juice of Italian ryegrass

Animal Science and Technology, Sichuan Agricultural University College, Ya’an 625000, China

Received Date: 2013-06-30
Publish Date: 2013-11-14

Abstract: In order to study lactic acid bacteria species which plays a major role in the fermentation process of Italian ryegrass(Lolium multiflorum) silage, 6 lactic acid bacteria strains(L-1, L-3, L-4, L-5, L-8 and L-9) were isolated from previously fermented juice of Changjiang No.2 Italian ryegrass. The results of traditional biochemical identification, experiment of acid-production rate, acid tolerance test, salt tolerance test, and the 16S rRNA sequence analysis indicated that L-1, L-3, L-4, L-5, L-8 and L-8 are Lactobacillus plantarum, Lactococcus lactis, Lactococcus garvieae, Leuconostoc lactis, Weissella confuse and Weissella cibaria, respectively, and all of them have good acid productivity, strong acid and salt tolerance. Further studies on using these strains as feed additives can be made.

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参考文献(1)

[1]

张子仪.中国饲料学.北京:中国农业出版社,2003:334.[3] 陈修鸾,曹成禹,李名远.常用饲料和牧草栽培技术.成都:四川科学技术出版社,1987:38-39.[4]于文,高升.绿汁发酵液对青贮发酵品质影响的研究进展[J].乳业科学与技术.2006,29(5):242-243[8]张涛,崔宗均,高丽娟,等.绿汁发酵液和乳酸菌剂MMD3在不同含水率苜蓿青贮中的添加试验[J].中国农业大学学报.2004,9(5):32-37[10] 刘晗璐.禾本科牧草乳酸菌发现及发酵品质检测与动物生产性能影响研究.呼和浩特:内蒙古农业大学,2008.[11] 凌代文,东秀珠.乳酸菌细菌分类鉴定及实验方法.北京:中国轻工业出版社,1999:33-115.[12]张艾青,刘书亮,敖灵.产广谱细菌素乳酸菌的筛选与鉴定[J].微生物学通报.2007,(4):753-756[17] 李晓霞.不同添加剂对八种牧草青贮效果的影响.甘肃:甘肃农业大学,2008.[18]张涛,崔宗均,李建平,等.不同发酵类型青贮菌制剂对青贮发酵的影响[J].草业学报.2005,14(3):67-71

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多花黑麦草绿汁发酵液中乳酸菌的分离鉴定

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Isolation and identification of lactic acid bacteria from previously fermented juice of Italian ryegrass

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